ABSTRACT Introduction: The objective of this research was to explore the association between the presence of depressive symptoms and the existence of symptoms suggestive of urinary incontinence in a group of women treated in a public hospital in Peru. Material and Methods: Cross-sectional study was conducted in women with “Seguro Integral de Salud” who attended the gynecology consultation room of Hospital Nacional Daniel Alcides Carrión during November and December of 2018. A sample was calculated with Epidat version 3.1 and the participants were selected by convenience. The main variables of interest were urinary incontinence and depression, both measured by validated measurement instruments: ICIQ-SF and self-assessment scale for Zung depression, respectively. Fisher's exact and Kruskall-Wallis were used to explore the association. A total of 186 women aged between 40 and 60 years were studied. Results: A total of 176 women (94,6%) defined themselves as incontinent and 183 as depression (98,4%). No association was found between the symptoms suggestive of depression with symptoms suggestive of urinary incontinence (p>0,05), but there was an association between the degree of depressive symptoms and the incontinence score (p = 0,0001). Conclusions: A considerable proportion of women suffer from depressive symptoms and urinary incontinence. On the other hand, there seems to be a possible association between urinary incontinence score and the severity of depressive symptoms. More studies are needed to explore this possible association to elucidate the impact that urinary incontinence has on the mood of women in Peru.

RESUMEN Introducción: El objetivo fue explorar la frecuencia y asociación entre la presencia de síntomas depresivos con la existencia de síntomas sugestivos de incontinencia urinaria en un grupo de mujeres atendidas en un hospital público del Perú. Material y Métodos: Estudio transversal realizado en mujeres con Seguro Integral de Salud que acudieron al consultorio de ginecología del hospital público de alta complejidad Hospital Nacional Daniel Alcides Carrión durante noviembre y diciembre del 2018. Se calculó una muestra con Epidat versión 3.1 y se seleccionó a las participantes por conveniencia. Las principales variables de interés fueron incontinencia urinaria y depresión, ambas medidas mediante instrumentos de medición validados: ICIQ-SF y escala de autoevaluación para la depresión de Zung, respectivamente. Para explorar la asociación se usó exacta de Fisher y Kruskall-Wallis. Resultados: Se estudió a 186 mujeres con edades entre 40 y 60 años. Un total de 176 mujeres (94,6 %) se definieron como incontinentes y 183 con depresión (98,4 %). No se encontró asociación entre los síntomas depresivos con los síntomas sugestivos de incontinencia urinaria (p>0,05), pero sí en el grado de síntomas depresivos con el puntaje de incontinencia (p=0,0001). Conclusiones: Una proporción considerable de mujeres padece de síntomas depresivos y de incontinencia urinaria. Por otra para, parece existir una posible asociación entre los puntajes de incontinencia urinaria y la severidad de los síntomas depresivos. Se requieren más estudios que exploren esta posible asociación para dilucidar el impacto potencial que la incontinencia urinaria posee sobre el estado anímico de las mujeres en Perú.

Introdução: A biopsia renal e fundamental na abordagem diagnostica e no prognostico de doentes com patologias nefrológicas. No sentido de avaliar a epidemiologia das doenças renais na Região Autónoma da Madeira e a sua evolução, analisou-se as biopsias de rim nativo nos ultimos vinte e sete anos. Métodos: Procedeu-se a análise retrospectiva dos registos e laminas histológicas de 1986-2012, com avaliação da evolução temporal na qualidade/indicações para biopsia renal e padrões de patologias nefrologicas. Resultados: A amostra compreende 315 biopsias de rim nativo, sendo a idade media dos doentes de 40.8 ± 18.4 anos, sendo 50.5% (n = 159) do sexo masculino. As síndromes nefrologicas mais frequentes foram a sindrome nefrotica (36.2%, n = 114) e lesão renal aguda (20.0%, n = 63). Das patologias nefrologicas mais frequentes, destacam-se no grupo das glomerulopatias primárias (41.5%, n = 115), a nefropatia de IgA (26.1%, n = 30) e a glomeruloesclerose segmentar e focal (17.4%, n = 20) e nas glomerulopatias secundarias (31.4%, n = 87), a nefrite lupica (51.7%, n = 45) e amiloidose (20.7%, n = 18). Na analise estatistica, realca-se a correlação significativa entre sexo e grupos de sindromes nefrologicas (Fisher’s exact test, p<.01) e entre grupos de sindromes nefrologicas e diagnostico patologicos (χ2, p <.01). Avaliando a evolução temporal, não se objectivaram diferencas estatisticamente significativas em relação ao numero de biopsias renais (χ2, p =.193), numero de glomerulos na amostra (Fisher’s exact test, p =.669), idade (Kruskal-Wallis, p =.216), sindromes nefrologicas (χ2, p =.106) ou grandes grupos de diagnostico patologicos (χ2, p =.649). No entanto, considerando os dignósticos patologicos especificos e a sua variação temporal foram encontradas diferencas significativas (Fisher’s exact test, p <.05) para nefrite lupica e nefropatia membranosa com uma incidencia crescente e para a amiloidose com uma tendência oposta. Discussão: A analise do registo de biopsias renais de uma população com características particulares, isolada geograficamente, permitiu mostrar concordância com outros números epidemiológicos referentes a outras subpopulações europeias, permitindo simultaneamente uma compreensão das directrizes locais sobre biopsias renais.

Introduction: Renal biopsy plays an essential role either in the diagnosis or in the prognosis of patients with renal disease. In order to assess its epidemiology and evolution in Madeira Islands, we analysed twenty-seven years of native kidney biopsies. Methods: We performed a retrospective analysis of clinical records, including histological revision from 1986 to 2012, totalling 315 native kidney biopsies. They were assessed regarding the temporal evolution both for the quality/indications for renal biopsy and for the patterns of kidney disease. Results: A total of 315 native kidney biopsies were analysed. The patients’ mean age was of 40.8 �± 18.4 years and 50.5%(n = 159) were males. The most common indications for renal biopsy were nephrotic syndrome (36.2%, n = 114) and acute kidney injury (20.0%, n = 63). Among primary glomerular diseases (41.5%, n = 115) the most common were IgA nephropathy (26.1%, n = 30) and focal-segmental glomerulosclerosis (17.4%, n = 20) and among secondary glomerular diseases (31.4%, n = 87), lupus nephritis (51.7%, n = 45) and amyloidosis (20.7%, n = 18). Statistical analysis revealed significant correlation between gender and major pathological diagnosis (Fisher’s exact test, p <.01) and between indications for renal biopsy and major pathological diagnosis (χ2, p <.01). Regarding the temporal evolution, no statistically significant differences were found in the number of renal biopsies (χ2, p =.193), number of glomeruli per sample (Fisher’s exact test, p =.669), age (Kruskal-Wallis, p =.216), indications for renal biopsy (χ2, p =.106) or major pathological diagnosis groups (χ2, p =.649). However, considering the specific clinico-pathological diagnoses and their temporal variation, a statistically significant difference (Fisher’s exact test, p <.05) was found for lupus nephritis and membranous nephropathy with an increasing incidence and for amyloidosis with an opposite tendency. Discussion: The review of the native kidney biopsies from a population with particular characteristics, geographically isolated, such as those from Madeira Islands, showed parallel between epidemiological numbers referring to other European subpopulations, allowing simultaneously a comprehensive approach to our renal biopsy policies

We analysed 16 variable number tandem repeats (VNTR) and three single-nucleotide polymorphisms (SNP) in Mycobacterium leprae present on 115 Ziehl-Neelsen (Z-N)-stained slides and in 51 skin biopsy samples derived from leprosy patients from Ceará (n = 23), Pernambuco (n = 41), Rio de Janeiro (n = 22) and Rondônia (RO) (n = 78). All skin biopsies yielded SNP-based genotypes, while 48 of the samples (94.1%) yielded complete VNTR genotypes. We evaluated two procedures for extracting M. leprae DNA from Z-N-stained slides: the first including Chelex and the other combining proteinase and sodium dodecyl sulfate. Of the 76 samples processed using the first procedure, 30.2% were positive for 16 or 15 VNTRs, whereas of the 39 samples processed using the second procedure, 28.2% yielded genotypes defined by at least 10 VNTRs. Combined VNTR and SNP analysis revealed large variability in genotypes, but a high prevalence of SNP genotype 4 in the Northeast Region of Brazil. Our observation of two samples from RO with an identical genotype and seven groups with similar genotypes, including four derived from residents of the same state or region, suggest a tendency to form groups according to the origin of the isolates. This study demonstrates the existence of geographically related M. leprae genotypes and that Z-N-stained slides are an alternative source for M. leprae genotyping.

Um evento extremo de chuva registrado na cidade de Salvador, Bahia, é investigado neste trabalho. Dados observacionais de superfície e altitude, dados em pontos de grade, imagens de satélite meteorológico e informações de desastres fornecidas pela Defesa Civil de Salvador (CODESAL) foram utilizados na análise. No período entre a meia noite e 3 horas da madrugada do dia 12 de junho de 2006, a precipitação acumulada foi de 51 mm. Um número elevado de ocorrências foi registrado pela CODESAL, notadamente deslizamentos de encosta. Ventos fracos em toda a troposfera e energia potencial convectiva disponível máxima de 3.233 J kg-1 foram diagnosticados em Salvador no dia 11 de junho, quinze horas antes do período de chuva forte. Áreas de convecção profunda caracterizaram esse evento provocado por uma onda que chegou à costa leste do Nordeste do Brasil, após se propagar para oeste sobre o Atlântico Sul com velocidade de fase estimada de 10 m s-1, conforme evidenciado no campo da componente meridional do vento no nível de 700 hPa e em imagens de satélite meteorológico.

An extreme rainfall event registered in the city of Salvador, Bahia, is investigated in this work. Observational surface and upper air data, gridpoint data, meteorological satellite images and disasters information provided by the Civil Defense of Salvador (CODESAL) were used in the analysis. During the period from midnight to 3 A.M. on 12 June 2006 51 mm accumulated rainfall was registered. A high number of disasters were registered by CODESAL, particularly landslides. Weak winds throughout the troposphere and maximum convective available potential energy of 3,233 J kg-1 were identified in Salvador on June 11th, fifteen hours before the heavy rainfall. Areas of deep convection characterized this event, favored by a wave that reached the eastern coast of Northeast Brazil after moving westward over the South Atlantic with an estimated phase speed of 10 m s-1, as seen on the meridional wind component field at the 700 hPa level and on meteorological satellite images.

Objective Assessing VNTR (variable-number tandem repeat) variability of Mycobacterium leprae from Colombian patients with and without prior treatment to identify potential sources of infection and to understand the patterns of disease transmission. Methodology This was a descriptive cross-sectional study where a convenience sample of biopsies was taken from 161 multibacillary leprosy patients; diagnosis and monitoring of the disease had been requested for these patients. DNA was extracted from M. leprae and standardised using the PCR technique for M. leprae VNTR, genotypes were established and different clusters grouped by unweighted pair group method with arithmetic mean (UPGMA). Results 22 different VNTR genotypes were found from 161 samples, of which 100 samples (62.1%) had a single u-VNTR genotype and the remaining genotypes were VNTR 17 (5.6%), VNTR 20 (4.3%), VNTR 18 (4.3%), VNTR 14 (4.3%) and VNTR 13 (3.7%), namely those forming groups or clusters. Conclusion This study showed that clones can be detected with varying degrees of virulence / aggressiveness by cluster analysis, implying the need for more monitoring programme activities which will result in a real decline in microorganism transmission.

Objetivo Evaluar la variabilidad de VNTR (variable-number tandem repeat) de Mycobacterium leprae de pacientes colombianos con y sin tratamiento previo para identificar posibles fuentes de infección y entender los patrones de transmisión de la enfermedad. Metodología Estudio transversal descriptivo, en donde mediante un muestreo electivo a conveniencia se tomaron 161 biopsias de pacientes multibacilares de lepra, que habían sido solicitadas para diagnóstico y seguimiento de la enfermedad, de las cuales se realizó extracción de ADN de M. leprae y usando la técnica de PCR para VNTRs de M. leprae estandarizada, se establecieron los genotipos y los diferentes clusters mediante el agrupamiento apareado UPGMA. Resultados En las 161 muestras totales se hallaron 22 genotipos VNTRs diferentes, de las cuales 100 muestras (62,1 %) pertenecían al genotipo único VNTRU, y de los genotipos restantes, los mayoritarios, es decir los que dieron lugar a formación de grupos o clusters fueron VNTR17 (5,6 %), VNTR20 (4,3 %), VNTR18 (4,3 %), VNTR14 (4,3 %) y VNTR13 (3,7 %). Conclusión En este estudio se evidencia por análisis de agrupamiento que se pueden detectar clones con diferente grado de virulencia/agresividad, lo cual implica la necesidad de incrementar varias de las actividades del programa de control que darán como resultado la verdadera disminución de la transmisión del microorganismo.

Objetivo Evaluar la variabilidad de VNTR (variable-number tandem repeat) de Mycobacterium leprae de pacientes colombianos con y sin tratamiento previo para identificar posibles fuentes de infección y entender los patrones de transmisión de la enfermedad. Metodología Estudio transversal descriptivo, en donde mediante un muestreo electivo a conveniencia se tomaron 161 biopsias de pacientes multibacilares de lepra, que habían sido solicitadas para diagnóstico y seguimiento de la enfermedad, de las cuales se realizó extracción de ADN de M. leprae y usando la técnica de PCR para VNTRs de M. leprae estandarizada, se establecieron los genotipos y los diferentes clusters mediante el agrupamiento apareado UPGMA. Resultados En las 161 muestras totales se hallaron 22 genotipos VNTRs diferentes, de las cuales 100 muestras (62,1 %) pertenecían al genotipo único VNTRU, y de los genotipos restantes, los mayoritarios, es decir los que dieron lugar a formación de grupos o clusters fueron VNTR17 (5,6 %), VNTR20 (4,3 %), VNTR18 (4,3 %), VNTR14 (4,3 %) y VNTR13 (3,7 %). Conclusión En este estudio se evidencia por análisis de agrupamiento que se pueden detectar clones con diferente grado de virulencia/agresividad, lo cual implica la necesidad de incrementar varias de las actividades del programa de control que darán como resultado la verdadera disminución de la transmisión del microorganismo.

Objective Assessing VNTR (variable-number tandem repeat) variability of Mycobacterium leprae from Colombian patients with and without prior treatment to identify potential sources of infection and to understand the patterns of disease transmission. Methodology This was a descriptive cross-sectional study where a convenience sample of biopsies was taken from 161 multibacillary leprosy patients; diagnosis and monitoring of the disease had been requested for these patients. DNA was extracted from M. leprae and standardised using the PCR technique for M. leprae VNTR, genotypes were established and different clusters grouped by unweighted pair group method with arithmetic mean (UPGMA). Results 22 different VNTR genotypes were found from 161 samples, of which 100 samples (62.1%) had a single u-VNTR genotype and the remaining genotypes were VNTR 17 (5.6%), VNTR 20 (4.3%), VNTR 18 (4.3%), VNTR 14 (4.3%) and VNTR 13 (3.7%), namely those forming groups or clusters. Conclusion This study showed that clones can be detected with varying degrees of virulence / aggressiveness by cluster analysis, implying the need for more monitoring programme activities which will result in a real decline in microorganism transmission.